However, conservationists have taken aim at the Queensland government announcement, saying the extra funding will do nothing to stop the greatest threat facing the reef - climate change.
The Queensland government has announced $330.5 million will be included in next week's budget announcement for reef protection, delivering a strategy with ambitious targets to reduce sediment, nutrients and pesticides by 2032.
It comes after a federal lifeline of almost $92 million was delivered as Australia looks to avoid an unwanted "in danger" ruling for the world-famous wonder.
Queensland's funding will back programs and research jobs to help landowners reduce run-off in a bid to improve the reef's health.
The government said funding would help meet water quality targets by cutting land-based pollution, including $5.1 million for reducing sediment and nutrient run-off in the Burnett, Mary River and Cape York regions.
More details are expected to be unveiled in the Queensland budget, set to be handed down on June 23.
The Great Barrier Reef supports 77,000 jobs and contributes about $9 billion annually to Australia's economy.
"This is doing more than ever before to preserve this icon, which is such an important part of our tourism economy and local jobs," Treasurer David Janetzki said in a statement.
Australia is sweating on UNESCO's draft verdict on the reef's health in July, hoping to avoid an "in danger" tag that would prove damaging to tourism.
The May federal budget provided $91.8 million for projects to lift water quality, ramp up monitoring and trial coral‑spawning techniques designed to help damaged reefs bounce back faster after bleaching events.
The Great Barrier Reef Marine Park Authority reported the tourist icon was hit hard again last summer by coral bleaching, cyclones and flooding, all of which are linked to higher atmospheric and water temperatures.
Conservationists took aim at the Queensland government for making no reference to climate change in Monday's announcement, despite its own website acknowledging it as "the most pervasive and persistent influence on the Great Barrier Reef".
The Australian Marine Conservation Society said that while measures to improve water quality would help improve the reef's resilience, it failed to address the underlying issue.
''This is one of the most important actions governments can take to strengthen the reef's health, but it must be accompanied by stronger action on climate change," the society's Dr Max Hirschfeld said.
Dr Hirschfeld said that Queensland had a legislated target to cut greenhouse gas emissions by 75 per cent by 2035.
However, he said recent government decisions, including removing the renewable energy target and delaying emissions reductions targets, made it hard to see how the climate target would be achieved.
Queensland's Environment Minister Andrew Powell has been contacted for comment.
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